Wednesday, February 6th: Multifocus microscopy using diffractive Fourier optics for fast, super-resolution 3D imaging in Biology

Event Date: 

Wednesday, February 6, 2019 - 1:00pm

Event Location: 

  • Elings Hall 1605

Event Contact: 

Professor Philip Lubin, Physics

  • Special Colloquium on Diffractive Optics for Biological and other applications

Aberration-corrected multifocus microscopy (MFM) is a unique 3D microscopy technique based on diffractive Fourier optics that allows fast, high-resolution and super-resolution imaging of living biological samples [Abrahamsson et al. Nat Methods 2013]. Using specially designed diffractive optical elements, we multiplex and refocus the emission light from a wide-field microscope objective so that it simultaneously forms an entire "focal stack" of images of the 3D specimen volume. This focal stack of images forms the 3D image and is recorded simultaneously, allowing very fast 3D data acquisition. MFM imaging systems can be home-built from off-the-shelf optical- and opto-mechanical components and a set of multifocus diffractive gratings. Software (written in MATLAB) to design multifocus gratings is freely available from my lab [Abrahamsson et al. BOE 2016]. My lab manufactures MFM optics hands-on in the UCSB NanoFab, an open access facility. Our MFM systems can capture up to 25 simultaneous focal planes at >100 Hz. We are currently applying MFM in biological research projects to study neural circuit function in various model organisms, and in super-resolution imaging of transcription in the nucleus of single cells.

Sara Abrahamsson, UCSC